Intra-Tumoral Delivery of Functional ID4 Protein via PCL/ Maltodextrin Nano-Particle Inhibits Prostate Cancer Growth

Korang -Yeboah, Maxwell, Mercer University
Patel, Divya, Clark Atlanta University
Morton, Derrick, Clark Atlanta University
Sharma, Pankaj
Gorantla, Yamini, Mercer University
Joshi, Jugal, Clark Atlanta University
Nagappan, Perri, Clark Atlanta University
Pallaniappan, Ravi, Mercer University
Chaudhary, Jaideep, Clark Atlanta University

2016-07-03

2010-2019

ID4, a helix loop helix transcriptional regulator has emerged as a tumor suppressor in prostate cancer. Epigenetic silencing of ID4 promotes prostate cancer whereas ectopic expression in prostate cancer cell lines blocks cancer phenotype. To directly investigate the anti-tumor property, full length human recombinant ID4 encapsulated in biodegradable Polycaprolactone/Maltodextrin (PCL-MD) nano-carrier was delivered to LNCaP cells in which the native ID4 was stably silenced (LNCaP(-)ID4). The cellular uptake of ID4 resulted in increased apoptosis, decreased proliferation and colony formation. Intratumoral delivery of PCL-MD ID4 into growing LNCaP(-)ID4 tumors in SCID mice significantly reduced the tumor volume compared to the tumors treated with chemotherapeutic Docetaxel. The study supports the feasibility of using nanocarrier encapsulated ID4 protein as a therapeutic. Mechanistically, ID4 may assimilate multiple regulatory pathways for example epigenetic re-programming, integration of multiple AR co-regulators or signaling pathways resulting in tumor suppressor activity of ID4.

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Oncotarget, Vol. 7, No. 42

Clark Atlanta University

10.18632/oncotarget.10953

http://hdl.handle.net/20.500.12322/cau.ir:2016_yeboah_maxwell_k

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