Fabrication of Bioactive Surfaces by Functionalization of Electroactive and Surface-Active Block Copolymers
Olubi, Omotunde, Center for Functional Nanoscale Materials, Department of Chemistry, Clark Atlanta University London, Laurisa, Center for Functional Nanoscale Materials, Department of Chemistry, Clark Atlanta University Sannigrahi, Biswajit, Center for Functional Nanoscale Materials, Department of Chemistry, Clark Atlanta University Williams, Michael, Department of Physics, Clark Atlanta University Khan, Israt M., Center for Functional Nanoscale Materials, Department of Chemistry, Clark Atlanta University
2014-01-01
2010-2019
Biofunctional block copolymers are becoming increasingly attractive materials as active components in biosensors and other nanoscale electronic devices. We have described two different classes of block copolymers with biofuctional properties. Biofunctionality for block copolymers is achieved through functionalization with appropriate biospecific ligands. We have synthesized block copolymers of electroactive poly(3-decylthiophene) and 2-hydroxyethyl methacrylate by atom transfer radical polymerization. The block copolymers were functionalized with the dinitrophenyl (DNP) groups, which are capable of binding to Immunoglobulin E (IgE) on cell surfaces. The block copolymers were shown to be redox active. Additionally, the triblock copolymer of ?, ?-bi-biotin (poly(ethylene oxide)-b-poly (styrene)-b-poly(ethylene oxide)) was also synthesized to study their capacity to bind fluorescently tagged avidin. The surface-active property of the poly(ethylene oxide) block improved the availability of the biotin functional groups on the polymer surfaces. Fluorescence microscopy observations confirm the specific binding of biotin with avidin.
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articles
Bioengineering
Clark Atlanta University
http://www.mdpi.com/2306-5354/1/3/134
10.3390/bioengineering1030134
http://hdl.handle.net/20.500.12322/cau.ir:2014_olubi
http://rightsstatements.org/vocab/InC/1.0/