LLW-3-6 and Celecoxib Impacts Growth in Prostate Cancer Cells and Subcellular Localization of COX-2
Yerokun, Tokunbo, Department of Biology, Spelman College Winfield, Leyte L., Department of Chemistry and Biochemistry, Spelman College
2014-10-21
2010-2019
The proliferation in human prostate carcinomas, PC3 and MDA-PCa-2b, was analyzed for cells treated with LLW-3-6 and celecoxib in the presence and absence of sulfasalazine. LLW-3-6 was more potent than celecoxib at mediating a dose-dependent reduction of viable PC3 cells. Co-treatment with a non-lethal dose of sulfasalazine diminished the potency of both drugs in this cell line. The effects of the drugs in MDA-PCa-2b cells were less significant than those observed in the PC3 cells. Localization of COX-2 in LLW-3-6- and CBX-treated PC3 cells is consistent with protein aggregation known for cells responding to stress stimuli. To complement this, an analysis of the theoretical binding interactions of LLW-3-6 was completed to illustrate the potential of LLW-3-6 to bind to COX-2 in a manner similar to that of celecoxib. Studies to further define the mechanism of action for LLW-3-6 are ongoing. KEYWORDS: Benzimidazole, Celecoxib, COX-2, Sulfasalazine, PC3, MDA-PCa 2b, Prostate Cancer, Docking, Focal adhesion
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Anticancer Research
Department of Chemistry and Biochemistry, Department of Biology
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4204802/
http://hdl.handle.net/20.500.12322/sc.fac.pubs:2014_yerokun_winfield
http://rightsstatements.org/vocab/InC/1.0/