Characterization of preadipocytes associated to metabolic disease in obesity

Johnson, Justice
Malagon, Maria M.
Oliver, Tiffany

2014

2010-2019

The goal of this study was to characterize how preadipocytes differentiate under conditions of obesity. Accordingly, adipocyte staining using oil red O was conducted using differentiating 3T3-L1 cells. The cytoskeleton increases and accelerates the process of adipogenesis. Therefore, cytoskeletal proteins septin 11, vimentin, tubulin, and actin were also extracted from adipocytes on day 0, 3, 6, 8, 10 of differentiation and examined via western blot. Results from the analysis verified the identity of each of the proteins being present in the cytoskeleton. The results from the adipocytes differentiate into mature adipocyte cells. Thus, these results have contributed largely to research on metabolic syndrome in obesity.

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application/pdf

conference proceedings

Enhancing Global Research and Education in STEM at Spelman College (G-STEM)

Spelman College

http://hdl.handle.net/20.500.12322/sc.gstem:2014_johnson_justice

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