Modifications of avian fructose-1, 6-bisphosphatase with butanedione, 1988
Simmons, Claire Marie
1980-1989
Modification of chicken liver fructose-1, 6-bisphosphatase with butanedione in borate buffer (pH 8.1) leads to desensitization of the enzyme to inhibition by AMP and fructose-2, 6-bisphosphate. Desensitization to AMP inhibition can be prevented if modification is carried out in the presence of AMP, while fructose-1, 6-bisphosphate and fructose-2, 6-bisphosphate can protect the enzyme against desensitization to inhibition by fructose-2, 6-bisphosphate. Since butanedione is a reagent highly specific for modification of arginine in proteins, it is considered that arginine may be present at both the AMP and fructose-2, 6-bisphosphate sites.
text
application/pdf
1988-07-01
thesis
Master of Science (MS)
Atlanta University
Chemistry
Johnson, Joe Han, Peter
Clark Atlanta University
Georgia--Atlanta
http://hdl.handle.net/20.500.12322/cau.td:1988_simmons_claire_m