The role of AP-1 proteins in the transforming growth factor-beta signaling pathway in prostate cancer, 2005
Russell, Daree E.
2000-2009
Transforming growth factor-beta (TGF-?) has a variety of roles including cell growth inhibition, stimulation of proliferation, differentiation, apoptosis and extracellular matrix (ECM) formation. TGF-? inhibits growth of the epithelial cells in the prostate gland. The ability of TGF-? to inhibit cell growth enables it to act as a potent tumor suppressor. Here, we show that TGF-? has different effects on proliferation in DU145 (TGF-?- responsive) and PC3M (TGF-? - resistant) prostate cancer cell lines. RT-PCR analysis and Real-time PCR data have determined the varying levels of AP-1 family members in both cell lines. In PC3M and DU145 cells, we have shown that TGF-p exerts opposite effects on JunD, cFos and Fra2 expression, suggesting that AP-1 family members may be involved in the differential effects of TGF-? in these cell lines. The present observation confirms an intact inhibitory role of TGF-? on proliferation in DU145 cells, and shows a non-inhibitory function in PC3M cell growth. In PC3M cells TGF-? may exert effects leading to the metastatic nature of this cell line.
text
application/pdf
2005-12-01
thesis
Master of Science (MS)
Clark Atlanta University
Biological Sciences
Shafiq Khan
Georgia--Atlanta
http://hdl.handle.net/20.500.12322/cau.td:2005_russell_daree_e