Enantiometric resulation of epinephrine-structural analogues by cyclodextrin electrokinetic capillary chromatography, 2001
Ponds, Tamice R.
2000-2009
It has become increasingly important to develop accurate and reliable techniques for the separation of chiral drugs. Separation of epinephrine and eight chiral structural analogues by Cyclodextrin Modified Electrokinetic Capillary Chromatography or CECC has been examined. Steady-state fluorescence measurements are used to categorize the complexing abilities o epinephrine with eleven native and derivatized cyclodextrins. Using the relative fluorescence intensity values, the drugs/cyclodextrin interactions are qualitatively categorized as strong, weak or moderate. This data is tabulated or each drug. CECC experiments using different combinations of these cyclodextrins were used to separate epinephrine as a model enantiomer. It is found that most of the cyclodextrin combinations that successfully separated the epinephrine enantiomers were cyclodextrins that either strongly and/or moderately interact with the drug as determined from steady state fluorescence measurements. This knowledge was used to predict apriori, the separation of the eight structural analogues of epinephrine. The results show good agreement between the predicted and the observed separation results.
text
application/pdf
2001-05-01
thesis
Master of Science (MS)
Clark Atlanta University
Chemistry
Anigbogu, Vincent
Georgia--Atlanta
http://hdl.handle.net/20.500.12322/cau.td:2001_ponds_tamice_r
