An investigation of BPDE-DNA adducts in t-lymphocytes from progeny mice exposed to benzo(a)pyrene during midgestation, 1996
Moolenaar, Pamela J.
1990-1999
Immune deficiency induced by benzo(a)pyrene (BP) in mice has been attributed to the metabolic intermediate benzopyrene-7,8-dihydrodiol-9,10-epoxide (BPDE). Our studies on mice progeny immune status after exposure of the pregnant female at midgestation (11-12 days) show a decided suppression in T-cell mediated immunity (CMI), reflected by deficiencies, particularly in the T-cell subsets L3T4 and Lyt 1. We hypothesize that this condition could also be a result of covalent binding of BPDE to DNA in T-lymphocytes (i.e., formation of BPDE-DNA adducts), which affect their functional status. New Zealand white rabbits were immunized with BPDE-DNA that was synthesized in vitro and conjugated to a strong immunogen, BSA, for the production of antibody against BPDE-DNA, confirmed by ELISA. Immunoaffinity purified anti-BPDE-DNA detected BPDE-DNA adducts in T-lymphocytes by ELISA and flow cytometric analysis. Finally, adduct positive cells were determined to have function when CM I was measured by the mixed response assay.
text
application/pdf
1996-05-01
dissertation
Doctor of Philosophy (PhD)
Clark Atlanta University
Department of Biological Sciences
Urso, Paul
Georgia--Atlanta
http://hdl.handle.net/20.500.12322/cau.td:1996_moolenaar_pamela_j
