In vivo and in vitro evidence for the synthesis and processing of a styrene oxide induced form of epoxide hydrolase, 1985

Wilson, Jewell D.

1980-1989

In vivo and in vitro studies have been done to determine the site of biosynthesis and processing of styrene oxide (SO) induced epoxide hydrolase (EH). Adult male LongEvans rats were used to induce a form of epoxide hydrolase using styrene oxide as the inducing agent. Rats were administered 500 milligrams of SO per milliliter of corn oil intraperitoneally per day. They were sacrificed forty-eight hours post-injections and the livers were quickly removed, homogenized and fractionated on a sucrose discontinuous density gradient to obtain the microsomal fraction. Epoxide Hydrolase was synthesized in a cellfree system using a rabbit reticulocyte lysate system. EH50 was partially purified using lubrol WX detergent solubilization and ion exchange chromatography. Analysis of EHsubS0 using SDS-PAGE showed the in vivo synthesis of a microsomal form of the enzyme. Autoradiography of the in vitro synthesis product (EHsubSO) showed that the enzyme is processed on RER-niembrane bound polysomes. The results suggest that EHsubS0 is synthesized on membrane-bound polysomes and follows the regulatory processing pathway of secretory proteins and,putatively, integral membrane proteins.

text

application/pdf

1985-07-01

dissertation

Doctor of Philosophy (PhD)

Atlanta University

School of Arts and Sciences, Biology

Browne, John M.

Clark Atlanta University

Georgia--Atlanta

http://hdl.handle.net/20.500.12322/cau.td:1985_wilson_jewell_d