Date of Award

7-1-1985

Degree Type

Dissertation

University or Center

Atlanta University (AU)

School

School of Arts and Sciences

Degree Name

Ph.D.

Department

Biology

First Advisor

Dr. John M. Browne

Abstract

In vivo and in vitro studies have been done to determine the site of biosynthesis and processing of styrene oxide (SO) induced epoxide hydrolase (EH). Adult male Long—Evans rats were used to induce a form of epoxide hydrolase using styrene oxide as the inducing agent. Rats were administered 500 milligrams of SO per milliliter of corn oil intraperitoneally per day. They were sacrificed forty-eight hours post-injections and the livers were quickly removed, homogenized and fractionated on a sucrose discontinuous density gradient to obtain the microsomal fraction. Epoxide Hydrolase was synthesized in a cell—free system using a rabbit reticulocyte lysate system. EH50 was partially purified using lubrol — WX detergent solubilization and ion exchange chromatography. Analysis of EHsubS0 using SDS-PAGE showed the in vivo synthesis of a microsomal form of the enzyme. Autoradiography of the in vitro synthesis product (EHsubSO) showed that the enzyme is processed on RER-niembrane bound polysomes. The results suggest that EHsubS0 is synthesized on membrane-bound polysomes and follows the regulatory processing pathway of secretory proteins and,putatively, integral membrane proteins.

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Biology Commons

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