Date of Award
University or Center
Atlanta University (AU)
Dr. Roy Hunter Jr.
This investigation was undertaken in order to analyze the microbial flora of two popular brands of commercial frozen-pack strawberries sold in major grocery stores in metropolitan Atlanta. The strawberries were diluted 1:1 and 10:1 and standard plate counts (SPC) were executed for a period of approximately five months. Tryptone glucose extract agar (TGE) and Sabouraud agar were the principal bacteriological media used throughout the plating procedure. The agar plates were incubated at 37 C and were observed for growth at 24 hr intervals for a period of 72 hr. The microorganisms that were found growing on the plates were isolated into pure cultures and subsequently subjected to a series of morphologi cal and biochemical analyses.
The principal fungus isolated was a yeast belonging to the genus Saccharomyces. No molds were isolated. The bacterial isolates were identified as Micrococcus tetragenus, Aerobacter aerogenes, Staphylococcus epidermidis, Proteus sp. and an unidentifiable species which was subjected to extensive studies for a period of one year. The results of these studies yielded evidence that was indicative of a hitherto undescribed genus and species. It was around this isolate that this investigation was primarily based.
White, Earl Jr, "Isolation and characterization of the microbial flora of frozen-pack strawberries" (1977). ETD Collection for AUC Robert W. Woodruff Library. 1230.