In vitro-analysis of the notochord of Rana Clamitans Larvae: the effects of l-azetidine-2-carboxylic acid, 1977
Sutton, Marilyn A.
1970-1979
The inhibitory action of L-azetidine-2-carboxylic acid (LACA) has been demonstrated by many investigators. Fowden and Richmond (1963), Takeuchi and Prockop (1969), Zamareava et al. (1969), Coulombre and Coulombre (1972), Aydelotte and Kochhar (1972) and many others have noted changes that occurred in the presence of this proline analog. Studies presented here are indicative of previous findings. Hematoxylin-eosin and Feulgen staining procedures afforded no distinguishable differences between experimental and control notochord fragments. Da Fano's method, spec ifically denoting Golgi zones (Humason, 1972), manifested an extreme increase in silver staining areas in LACAtreated fragments, thereby suggesting an increased amount of activity in the Golgi or Golgi-containing areas. The incubation time appeared as an indicator of increasing staining. Electron microscopic examinations of the fragments correlated with those of light microscopic examinations. LACA-treated fragments as opposed to control fragments displayed numerous vesiculated areas. Often times electron- dense material was clearly noted in the vesicles. The cytoplasm of LACA-treated fragments also contained more fibrils than proline-treated or control fragments. Mitochondria and other electron-dense bodies showed no differences in experimentals or controls. The LACA-treated fragments displayed cell membrane interruptions which were not observed in proline-treated or control fragments. Occasionally fibrils extending to the extracellular matrix were noted in LACA-treated fragments. These findings suggest that LACA is interferring with the cell activities of protein packaging and extrusion, particularly collagen.
text
application/pdf
1977-08-01
thesis
Master of Science (MS)
Atlanta University
Biology
Hunter, Roy
Clark Atlanta University
Georgia--Atlanta
http://hdl.handle.net/20.500.12322/cau.td:1977_sutton_marilyn_a
