Date of Award

12-1-1988

Degree Type

Dissertation

University or Center

Atlanta University (AU)

Degree Name

Ph.D.

Department

Biology

First Advisor

Dr. Clarence W. Clark

Abstract

The interactions of plasmid DNA with the membranes of Proteus mirabilis has been investigated under in vitro conditions. Analysis of the endogenous plasmid, NR1, the compatible plasmid, R6K, and the incompatible plasmid, R6- 5, on 5-20% neutral sucrose gradients, following the in vitro binding assay revealed binding to inner and outer membrane preparations. In competition experiments, R6K did not compete with NR1 for binding site(s). However, R6-5 successfully competed with NR1. Furthermore, high salts (0.2 M KCl or MgC12) inhibited the binding of NR1, R6K, and R6-5 to the membranes of P. mirabilis. However, 5-20% gradients containing salts did not release plasmids from their bound templates. In addition, plasmid minus P. mirabilis membranes did not bind compatible nor incompatible plasmids. Restriction enzyme digested fragments did not bind to membrane fractions.

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Biology Commons

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