Date of Award

7-1-1988

Degree Type

Thesis

University or Center

Atlanta University (AU)

Degree Name

M.S.

Department

Chemistry

First Advisor

Dr. Joe Johnson

Second Advisor

Dr. Peter Han

Abstract

Modification of chicken liver fructose-1, 6-bisphosphatase with butanedione in borate buffer (pH 8.1) leads to desensitization of the enzyme to inhibition by AMP and fructose-2, 6-bisphosphate. Desensitization to AMP inhibition can be prevented if modification is carried out in the presence of AMP, while fructose-1, 6-bisphosphate and fructose-2, 6-bisphosphate can protect the enzyme against desensitization to inhibition by fructose-2, 6-bisphosphate. Since butanedione is a reagent highly specific for modification of arginine in proteins, it is considered that arginine may be present at both the AMP and fructose-2, 6-bisphosphate sites.

Included in

Chemistry Commons

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