Date of Award


Degree Type


University or Center

Atlanta University (AU)

Degree Name




First Advisor

Dr. John M. Browne


The lens crystallins have been utilized by a variety of investigators who are interested in the biochemical differentiation of the vertebrate lens, Dimethyl Sulfoxide (DMSO) is known to be very effective in altering the configuration of proteins. Synthesis of the crystallins may be altered by the use of DMSO. It was the purpose of this investigation to determine if alterations do occur and if so in what manner do these alterations affect the developing eye of rat fetuses of the Long-Evans strain. Lenses from fetuses of normal and DMSO-treated maternal rats showed marked changes in protein concentration and moieties. The greatest increase resulted when maternal rats were treated with DMSO. Densitometric analyses of polyacrylamide gel fractions further show that DMSO does cause changes in lens protein concentrations during late rat fetalogenesis. Because of these observed anomalies in lens protein, further characterization in the fractions for the determination of the effects on specific crystallins is warranted.

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