Date of Award

11-1-2012

Degree Type

Thesis

University or Center

Clark Atlanta University(CAU)

School

School of Arts and Sciences

Degree Name

M.S.

Department

Chemistry

First Advisor

Dr. Eric A. Mintz

Second Advisor

Dr. Roosevelt Thedford

Third Advisor

Dr. David Logan

Abstract

The inactivation of E. coli K12 (ATCC 27325) and the changes in protein content and protein oxidation during the photocatalytic inactivation with titanium oxide, Degussa P25, was investigated under long wavelength U’! (365 nm) light. The bacterial suspensions were challenged with UV light (365 nm) for 0, 15, 30, and 60 mm, samples collected serially diluted, plated, incubated and enumerated to determine rate of inactivation. The protein was recovered from these bacterial suspensions by centrifugation and the protein content determined by the Lower method and the extent of oxidation determined by treatment with 2,4-dinitorphenyihydrazien and measuring the absorbance at 427 nm. The recovered proteins were also characterized by sodium dodecyl sulfate poly acrylamide gel electrophoresis (SDA PAGE) and Western Blotting. The decrease in protein content and degree of protein oxidation was found to parallel cell inactivation.

Signature Location_Supplemental file.pdf (45 kB)
Notice to Users, Transmittal and Statement of Understanding

Included in

Chemistry Commons

Share

COinS