Date of Award


Degree Type


University or Center

Clark Atlanta University(CAU)


School of Arts and Sciences

Degree Name




First Advisor

Dr. David B. Cooke, III


Tumor biologist have long appreciated that both cell to

cell and cell to extracellular matrix (ECM) interactions are

involved in the invasive and metastatic events that are

characteristic of malignancy. Cancer cell attachment to and

invasion of an ECM has been associated with metastatic

potential of cell lines of the Dunning rat prostate model.

It was postulated that differences observed in the

metastatic potential of four Dunning cell lines may

correlate with cell-matrix interactions. Four cell lines,

highly metastatic ML, MLL, AT-3 and non-metastatic AT-1 were

studied. The adhesive, invasive and chemoinvasive

capability of each cell line was compared. Cell adhesion

was examined by plating the cells on plastic dishes coated

with various components of the ECM (fibronectin, laminin and

collagen) as well as EHS Natrix (a natural ECM) . Invasion

was determined by examining cells ability to traverse a

matrigel barrier. Correlations were found between the

cells' adhesive and invasive abilities in response to the

ECM. These observations suggest that ECM components are

highly involved in prostate cancer cell activities and loss

may contribute to tumor progression and metastasis.


Signature pages are on file with the graduate school. An archival copy of the document is available in the Archives Research center.