Induction of alkaline phosphatase during lymphocyte blast transformation in cba mice, 1979
Merritt, Brenda E.
1970-1979
Alkaline phosphatase is a membrane-bound enzyme which hydrolyzes phosphate esters at an alkaline pH. This enzyme has been found localized on the murine thymic lymphoblast, in the placenta and embryonic thymus up to 16 day gestation. Alkaline phosphatase has also been found in cells surrounding the germinal centers in the normal adult spleen of C57B1/6J mice. Lymphocytes from the thymus, nodes, and spleen of normal adult CBA mice were stimulated with concanavalin A to determine the appearance of alkaline phosphatase during dedifferentiation of the normal immune response and to determine the macromolecular requirements needed for this enzyme induction. Fluorometric analysis of time course experiments of blasting lymphocytes indicated that alkaline phosphatase does appear in blasting lymphocytes. Mitomycin C inhibited DNA synthesis and alkaline phosphatase induction. Actinomycin D inhibited both RNA and DNA synthesis and alkaline phosphatase induction. Therefore, the induction of alkaline phosphatase may be used as a lymphoblastic marker in studying dedifferentiation in the normal immune response.
text
application/pdf
1979-08-01
thesis
Master of Science (MS)
Atlanta University
Department of Biology
Lumb, Judith Rae
Clark Atlanta University
Georgia--Atlanta
http://hdl.handle.net/20.500.12322/cau.td:1979_merritt_brenda_e