Fluorescence studies of bovine liver fructose-1.6-diphosphatase, 1988

Moleli, Lebona E.

1980-1989

Bovine liver fructose-1,6-diphosphatase was purified to homogeneity. Fluorescense studies were used to probe the structure of the active site of the enzyme. Fluorescense probe N-( 1-pyrene) maleimide (NTM) is nonfluorescent in aqueous solution but forms a strong fluorescent adduct with the enzyme through its essential sulfhydryl group. The effect of pH, ionic strength, urea, AMP and FDP on environment of the essential sulfhydryl group as measured by the fluorescent probe was studied.

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1988-12-01

thesis

Master of Science (MS)

Atlanta University

Department of Chemistry

Zeidan, Henry M.

Clark Atlanta University

Georgia--Atlanta

http://hdl.handle.net/20.500.12322/cau.td:1988_moleli_lebona_e