Date of Award

12-1-1993

Degree Type

Thesis

Degree Name

M.S.

Department

Department of Biological Sciences

First Advisor

Dr. Judith Bender

Abstract

A chlordane-tolerant strain of cyanobacteria was developed. The cyanobacteria was immobilized on ensiled grass clippings which provided nutrients and added a number of microaerophilic bacteria to the system. Subcultures of this Silage Microbial Mat were treated with 200 mg/1 of chlordane. Significant levels of chlordane disappearance were observed in five days. Ensiling bacteria, Chromatia, and Oscillatoria isolated from this system also demonstrated the ability to degrade chlordane. HPLC, GC/MS and an invertebrate bioassay confirms that the process was biodegradation and not bioaccumulation. This research thus provides an archetype for much needed remediation technology.

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