Date of Award
Department of Biology
Dr. William LeFlore
Sera from rabbits which were injected with a lyophilized antigen of whole Cysticercus fasciolaris worms were tested for antibodies using the microprecipitin, agar double diffusion, Immunoelectrophoresis and indirect hemagglutination tests. Each test gave a positive reaction for antibodies; however, the indirect hemmagglutination test appeared to be more sensitive in that the titer was higher than the others. These antisera were used in performing immunohistochemical studies which consisted of the indirect fluorescent antibody (IFA) technique and the horseradish peroxidase (HRP) method. The pattern and intensity of staining obtained with the IFA technique and the HRP technique were compared. It was observed that the HRP technique produced specific positive reactions at a higher titer than the IFA. An advantage of this technique as compared to the IFA technique is that it could be readily evaluated with a conventional microscope. It is suggested that the HRP technique should provide a satisfactory alternative to the IFA in the diagnosis of cysticercosis.
Ndifor, Fombotioh, "Immunohistochemistry of cysticercus fasciolaris using fluorescein and horseradish peroxidase-labeled conjugates: a comparison of the two techniques" (1977). ETD Collection for AUC Robert W. Woodruff Library. 3762.