Date of Award
7-1-2005
Degree Type
Thesis
University or Center
Clark Atlanta University(CAU)
School
School of Arts and Sciences
Degree Name
M.S.
Department
Biology
First Advisor
Dr. Juarine Stewart
Second Advisor
Dr. John Browne
Third Advisor
Dr. Alfred Merrill
Recommended Citation
Banks, Mara E., "Identification of mechanism(s) by which the complex sphingolipid, C2-ceramide, influences CYPIA1 induction fy 3-Methylcholanthrene" (2005). ETD Collection for Robert W. Woodruff Library, Atlanta University Center. Paper 49.
http://digitalcommons.auctr.edu/dissertations/49
COinS
Comments
Sphingolipids facilitate cell growth, differentiation and signaling. P450s, in general, aid in xenobiotic transformation, vascular autoregulation in the brain and the formation of sterols like cholesterol and steroids. CYPl A1 acts on polycyclic aromatic hydrocarbons making them more soluble and easier for cell secretion. Earlier studies in this laboratory had found that the complex sphingolipid, C2-ceramide, modulates CYPl A1 induction by 3-Methylcholanthrene. Using Western Blot analysis, confocal microscopy, and Electrophoretic Mobility Shift Assays, we have determined the mechanism C2-ceramide uses for this modulation. Electrophoretic Mobility Shift Assays and Western Blots Analysis revealed no significant change in 3MC-AhR-ARNT triplex binding to cypl a1 XREl or AhR and ARNT protein concentrations in the presence of C2-cerarnide, respectively. It is the ability of C2-ceramide to form large stable pores in the plasma membrane, allowing more 3MC to enter, that modulates CYPlAl induction by 3MC.