Date of Award


Degree Type


University or Center

Clark Atlanta University(CAU)


School of Arts and Sciences

Degree Name




First Advisor

Dr. Juarine Stewart

Second Advisor

Dr. John Browne

Third Advisor

Dr. Alfred Merrill


Sphingolipids facilitate cell growth, differentiation and signaling. P450s, in general, aid in xenobiotic transformation, vascular autoregulation in the brain and the formation of sterols like cholesterol and steroids. CYPl A1 acts on polycyclic aromatic hydrocarbons making them more soluble and easier for cell secretion. Earlier studies in this laboratory had found that the complex sphingolipid, C2-ceramide, modulates CYPl A1 induction by 3-Methylcholanthrene. Using Western Blot analysis, confocal microscopy, and Electrophoretic Mobility Shift Assays, we have determined the mechanism C2-ceramide uses for this modulation. Electrophoretic Mobility Shift Assays and Western Blots Analysis revealed no significant change in 3MC-AhR-ARNT triplex binding to cypl a1 XREl or AhR and ARNT protein concentrations in the presence of C2-cerarnide, respectively. It is the ability of C2-ceramide to form large stable pores in the plasma membrane, allowing more 3MC to enter, that modulates CYPlAl induction by 3MC.

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