Chemical dissolution and in vitro studies of 2-day old chick embryo notochordal cells, 1964

Williams, Richard Ernie

1960-1969

This study was undertaken. to determine the behavioral pattern of dissociated 2-day old chick notochordal. cells in vitro. Notochords were dissected from embryos that had been incubated for 48 hours (1820 somites) at 37.50 degrees celsius and complete ly dissociated in a 2% tetrasodium ethylene diamine tetra acetic acid/calcium and magnesiumfree NiuTwitty- solution. Pieces of notochordal tissue and dissociated cells were separately cultured in a fluid medium (1% dextrose/ca1cium~ and magnesium-.free NiuTwitty solution) by the lying drop single coverslip technique. Extensive outgrowth from the explant, cell division, and changes in cell shape were Observed in the cultured tissue fragments. The dissociated cells in culture aggregated to form clumps that possessed loose cell attachments. No cell division or noticeable change in cell shape, however, occurred in these cells. It was concluded in light of these observations that calcium and/or magnesium are intimately involved in the role of cell adhesion in the 2day old chick notochord. Also, a 1% dextrose/calcium- and magnesiuium-free culture medium does not support mitotic activity of single notochordal cells of the same age.

text

application/pdf

1964-05-01

thesis

Master of Science (MS)

Atlanta University

Biology

Hunter, Roy

Clark Atlanta University

Georgia--Atlanta

http://hdl.handle.net/20.500.12322/cau.td:1964_williams_richard_e