A modern fish known as the African Coelacanth, latimeria chalumnae or the “living fossil” is seen to closely resembles the fossilized skeletons of its ancient 300-million-year-old relatives. One important characteristic noted about the coelacanth was its lack of Immunoblobin-M (IgM). One possible link to this observation may be due to the role of the lectin super family for immunity. Recent gene duplication events were found in the C-type lectin protein families of the coelacanth. The superfamily of protein containing lectins known for their immunity abilities are referred to as C-type lectin –like domains (CTLD). CTLD are a large group of extracellular metazoan, proteins with diverse roles. The ensembl database was used to search for information about the various Celocanth genes, so that the genes could be manually curate through annotation. A database search was done for the 81 C-type lectin genes found within the latimeria chalumnae (L.C.) to be compared to 10 C-type lectin genes found in the takifugu rubripes (T.R.) and 10 found in homo sapiens (H.S.). In order to compare the Latimeria chalumnae (L.C.) genomes, an analysis of the orthologous groups (OG) number of exons, superfamily domain, print domain, start/stop location, protein length and split variants was examined. It is noted that there is a conserved number of exons within the L.C. to be 3-7. The typical splice variants for these genes are one and the average protein length is a 100-1000aa. The majority of the print domains for the Latimeria chalumnae are seen to be Antifreezell. The large abundance of these antifrezell proteins are not only seen in the L.C., but also the Takigufu rubripes as well. The orthologous group of this organism were seen to have a much higher splice variants and higher locations start and stop locations. It is also noted that print domain of the L.C. was not available for a large amount of the genes. The domain of these genes may have slightly evolved, yet still retains the overall structure to the c-type lectin. In the future, the Latimeria Chalummnae C-type Lectin genes will undergo BLASTing to be further examined through a complete sequence alignmentA phylogenetic tree will be constructed to further understand the C-type Lectin genes within organism such as the Latimeria Chalummnae.
Coleman, Niwa; Winfield, Leyte; and Christoffels, Alan, "Analysis of C-type Lectin – Like Domains of the African Coelacanth Genome" (2013). G-STEM Posters. 13.