Document Type

Conference Proceeding

Publication Date

Summer 2016

Abstract

The study focuses on the microbial content of four different freshwater sources in Glasgow Scotland. The sources are Kelvin Grove Fountain, Kelvin River, Loch Ness, and a pond near Fort Augustus known as Jenny’s Pond. The bacteria in the water were grown, isolated, gram stained, endospore stained, and sequenced using the 16s rRNA. Pollution levels were determined using a colony count ratio grown at 22C (water temperature) and 37C (body temperature).The bacteria were placed on Blood, MacConkey, Mannitol Salt, and Eosin Methylene blue Agar. Also viruses were tested using bacteriophage plaque assay. The bacteriophage assay made with the filtered Kelvin water and bacteria DS902, showed 13 plaques on the nutrient agar plate. However, there were no plaques present from the chosen bacteria samples from the Kelvin River. There were also no plaques present from the remaining three water sources. The Kelvin Grove Water Fountain and Loch Ness were not polluted water sources. However, the Kelvin River, Cyprus Duck pond and Jenny’s pond were polluted according to the ratio calculated. The Kelvin Grove Fountain had the highest ratio, which was 17.5. Loch Ness also had a high ratio of 13.21. These water sources contained several pathogenic bacteria. The Kelvin River had the lowest ratio, which was 0.8. Jenny’s Pond had a low ratio of 5.

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