Document Type

Article

Publication Date

2017

Department

Biology,Department of Biological Sciences

Abstract

Sex is beneficial in eukaryotes as it can increase genetic diversity, reshuffle their genomes, and purge deleterious mutations. Yet, its evolution remains amystery. The eukaryotic clade supergroup Amoebozoa encompasses diverse lineages of polymorphic amoeboid forms, including both free-living and parasitic lineages. The group is generally believed to be asexual, though recent studies show that someof itsmembers are implicated in cryptic forms of sexual cycles. In this study,weconduct a comprehensive inventory and analysis of genes involved in meiosis and related processes, in order to investigate the evolutionary history of sex in the clade. We analyzed genomic and transcriptomic data of 39 amoebozoans representing all major subclades of Amoebozoa. Our results show that Amoebozoa possess most of the genes exclusive to meiosis but lack genes encoding synaptonemal complex (SC). The absence of SC genes is discussed in the context of earlier studies that reported ultrastructural evidence of SC in some amoebae. We also find interclade and intrageneric variation in sex gene distribution, indicating diversity in sexual pathways in the group. Particularly, members of Mycetozoa engage in a novel sexual pathway independent of the universally conserved meiosis initiator gene, SPO11. Our findings strongly suggest that not only do amoebozoans possess sex genes in their genomes, but also, based on the transcriptome evidence, the present sex genes are functional.We conclude that Amoebozoa is ancestrally sexual, contrary to the long held belief that most of itsmembers are asexual. Thus, asexuality inAmoebozoa, if confirmed to be present, is a derived-trait that appeared later in their evolution.

DOI

10.1093/gbe/evx002.

Source

Genome Biology and Evolution 9, no. 2 (2017): 375-387

Included in

Life Sciences Commons

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